关键词: 小鼠肝瘤H22

Abstract: With the single cell cloning technique, we cloned H2D8 subline from H 22 cell line stored by China Center for Type Culture Collection in Wuhan (Wuhan H 22 ). Then the comparative analysis of Wuhan H 22 and H2D8 about their dependence on serum when cultured in vitro , growth feature in vivo , pathomorphism and therapeutic feature to CTX were carried on. With the results, we found that there was no significant difference between H2D8 and Wuhan H 22 about the dependence on serum, growth feature in vivo and pathomorphism. When the mice bearing tumor were treated with CTX,the restrain neoplastic rates of H2D8 was higher than that of Wuhan H 22 . It means that H2D8 clonal cell line retained the characteristics of Wuhan H 22 cell lines about the dependence on serum when cultured in vitro , grouwth feature in vivo and pathomorphism. H2D8 has higher sensitivity on the chemotherapy feature to CTX than that of Wuhan H 22 .

Key words: Murine Hepatoma 22, Biological characteristic, Chemotherapy