关键词: 两次打击, 急性肺损伤, Wistar大鼠, NF-kBp65mRNA

Abstract: Objective To observe the change of nuclear factor-kappa B(NF-kB) p65 mRNA expression in the lung tissue with acute lung injury(ALI) induced by oleic acid(OA) and lipopolysaccharide(LPS) in rats,and to investigate the relationship between nuclear factor kappa B p65 and ALI.Methods OA(0.2ml/kg) and LPS(2mg/kg) were injected intravenously to Wistar rats to develop ALI.The lung index(LI),WBC count,and percentage of ploymorphonuclear leucocyte(PMN) were observed,and the expression level of NF-kB p65 mRNA in the lung tissue with ALI was determined by situ hybridization technique.Results WBC in peripheral blood,and lung index of the rats with ALI caused by the 'two hits' were significantly increased,and PMN percentage changed markedly with the significant decrease in neutrophils and the significant increase in lymphocytes.The pathological sections of the lung showed an inflammatory change.The expression of NF-KB p65 mRNA in the lung tissue of the rats with ALI was markedly enhanced. Conclusion The enhanced expression of NF-kB p65 mRNA may make important contributions to development of ALI by 'Two hits'.

Key words: Two hits, Acute lung injury, Wistar rats, NF-kB p65 mRNA