实验动物科学

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血虚证动物模型的标准化研究初探

杨岚;祝彼得;彭成;   

  1. 成都中医药大学解剖组胚教研室,成都中医药大学,成都中医药大学科技处 成都610072,成都610072,成都610072
  • 收稿日期:2006-03-25 修回日期:2006-03-25 出版日期:2006-03-25 发布日期:2006-03-25

Study on Developing the Standard Animal Model of Blood Deficiency Syndrome

YANG Lan,ZHU Bi-de,PENG Cheng   

  1. (Chengdu University of Traditional Chinese Medicine,Chengdu 610072,China
  • Received:2006-03-25 Revised:2006-03-25 Online:2006-03-25 Published:2006-03-25

摘要: 目的建立标准化的血虚证动物模型。方法采用慢性失血法、溶血法和骨髓抑制法制造三种血虚证模型,观察一般情况和与血发生有关的实验室指标,并用四物汤反证造摸效果。结果三种动物模型均符合血虚证表现,同时出现外周血、骨髓有核细胞数目和细胞周期的改变,四物汤对三种动物模型均有一定疗效。结论建立了三种符合标准化的血虚证模型。

关键词: 血虚证, 动物模型

Abstract: Objective To develop the standard animal models of blood deficiency syndrome.Methods Three kinds of blood deficiency syndrome models were developed by blood loss,hemolysis,and arrest of bone marrow,and the symptoms,signs,and laboratory indexes related to hematogenesis were observed.The therapeutic effects of Siwutang(四物汤) were observed as counterevidence in three kinds of model. Results The manifestations of the animals in the three kinds of model were in keeping with clinical manifestations of blood deficiency syndrome, with the changes of peripheral blood,marrow cells and their cell-division period,and Siwutang(四物汤) could exert satisfactory therapeutic effects on the changes.Conclusion Three kinds of standard animal model of blood deficiency syndrome were established in mice.

Key words: Blood deficiency syndrome, Animal model