淋巴细胞脉络丛脑膜炎病毒(LCMV)PCR检测方法的建立与初步应用

实验动物科学

淋巴细胞脉络丛脑膜炎病毒(LCMV)PCR检测方法的建立与初步应用

邢进;卫礼;巩薇;贺争鸣;邢瑞昌;

中国药品生物制品检定所国家实验动物微生物遗传检测中心,中国药品生物制品检定所国家实验动物微生物遗传检测中心,中国药品生物制品检定所国家实验动物微生物遗传检测中心,中国药品生物制品检定所国家实验动物微生物遗传检测中心,中国药品生物制品检定所国家实验动物微生物遗传检测中心北京100050,北京100050,北京100050,北京100050,北京100050

收稿日期:2006-09-25 修回日期:2006-09-25 出版日期:2006-09-25 发布日期:2006-09-25

Establishment and Application of Assay for the Detection of LCMV Nested Polymerase Chain Reaction

XING Jin,WEI Li,GONG Wei,HE Zheng-ming,XING Rui-chang

(National Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050,China

Received:2006-09-25 Revised:2006-09-25 Online:2006-09-25 Published:2006-09-25

摘要/Abstract

摘要： 目的建立检测淋巴细胞脉络丛脑膜炎病毒(LCMV)的PCR方法。方法根据LCMV CDNA S片段的GPC和NP基因设计合成4对引物,对标准毒株进行套式PCR扩增并克隆测序,并对15份已知阳性或阴性小鼠鼠脑进行检测。结果目的片断与LCMV cDNA序列99%同源,对鼠脑的检测结果为10只阳性,5只阴性。结论检测小鼠鼠脑LCMV的结果准确,证明套式PCR能够应用于LCMV的实验室检测。

关键词: 淋巴细胞脉络丛脑膜炎病毒, 套式PCR

Abstract: Objective\ To establish PCR test method that monitor Lymphocytic Choriomeningitis Virus in Laboratory.Methods Design two series primers of Nested PCR according to S segment(GPC and NP genes) of LCMV cDNA.To clone and measure sequence for our LCMV strain though PCR.Use Nested PCR to test 15 mice brains.Results That is 99% homology between the target segment and LCMV cDNA.The test result of mice brains PCR is 10 positive and 5 negative.Conclusion The test result is correct on LCMV infected mice brains,therefore,it indicates the Nested_PCR method can be used for testing LCMV in Laboratory.

Key words: LCMV, Nested-PCR, Mouse brain

邢进;卫礼;巩薇;贺争鸣;邢瑞昌;. 淋巴细胞脉络丛脑膜炎病毒(LCMV)PCR检测方法的建立与初步应用[J]. 实验动物科学.

XING Jin,WEI Li,GONG Wei,HE Zheng-ming,XING Rui-chang. Establishment and Application of Assay for the Detection of LCMV Nested Polymerase Chain Reaction[J]. laboratory animal science.

[1]	佟巍;乔红伟;魏强;张丽芳;代小伟;刘先菊;王艳荣;刘云波;. 淋巴细胞脉络丛脑膜炎病毒在实验大鼠中的感染状况[J]. 实验动物科学, 2010, 27(04): 46-48.
[2]	邢进;肖镜;王吉;贺争鸣;. 套式PCR检测淋巴细胞脉络丛脑膜炎病毒(LCMV)方法应用[J]. 实验动物科学, 2008, 25(05): 53-55.