关键词: 乙型脑炎病毒, 单克隆抗体, 杂交瘤细胞

Abstract: Objective\ To prepare Monoclonal Antibodies against JEV and apply preliminary the antibodies to the detection.Methods\ BALB/c mice were immunized with purified Japanese Encephalitis Virus(JEV).The immunized spleen cells of these mice were fusing with SP2/0 myeloma cells.Four hybridoma secreting monoclonal antibodies(McAbs) against JEV were prepared after screening by ELISA and cloning three times by limiting dilution.Results\ Four hybridomas were obtained,named A5,Gl0,F4,and C10 respectively,which were able to secrete McAbs stably.The immunoglobulin subclass of G10 and F4 were IgG1 and A5 was IgM,the subclass of C10 was not identified;the ELISA titers of C10 and F4 ascites were above 10~5,while the ELISA and IFA titers of C10 and A5 ascites were above 10~2.Conclusion\ Monoclonal Antibodies against JEV were successfully prepared,the method of IFA and ELISA based on McAb was developed for the detection of JEV in the biological products from the swine for human use.

Key words: Japanese Encephalitis Virus(JEV), Monoclonal antibody, Hybridoma