关键词: 猪圆环病毒2型, ORF2基因, 原核表达

Abstract: Based on the porcine circovirus type 2(PCV2)LC strain,5 pair of primers were designed according to the Luchuan sequence,the ORF2,ORF2a,ORF2b,ORF2c and ORF2d gene were amplified from the PCV2-LC plasmids by PCR,and inserted into the prokaryotic expression vector PET-32a.The constructed recombinant expression plasmids were transformed into the receptive cells of E.coli BL21 and induced by IPTG with a finial concentration 1 mmol/L,then the recombinant protein was expressed in the form of inclusion bodies.The protein expression could react with PCV2 positive serum by Western-blot,sharing a good antigenicity.This study will provide a good fundament for development of PCV2 diagnostic ELISA diagnosis assay kit.

Key words: Porcine circovirus type 2, ORF2 gene, Prokaryotic expression