关键词: 重组人睫状神经因子, 谷氨酸钠肥胖大鼠, 降脂作用, 降血糖作用

Abstract: Objective To observe the antilipemic effects of recombinant human ciliary neurotrophic factor(rhCNTF) in the Neonatally Monosodium Glutamate-Treated Obesity model rats.Methods MSG obese rat model was established in rats by treatment with monosodium glutamate in the neonatal stage.The increases in the Lee's index(body weight1/3/body length) and in retroperitoneal fat as well as decreases in spontaneous motor activity were recorded.Food consumption and associated hypothalamic low body length obesity(monosodium glutamate-treated obesity;MSG-OB) were observed as these rats grew.Treatment with rhCNTF at 30～300 μg·kg-1s.c.once a day was given continuously for 33 days.The body weight and food intake were monitored every day.After final administration,rats were narcotized with choral hydrate,then the body length and weight were measured to calculate Lee's index.Blood was sampled through abdominal aorta to meature the serum levels of glucose and lipids.Fat around kidney and genitals and in abdominal cavity was collected for calculation of fat index.Results The body weight and Lee's index of MSG-rats significantly higher than that of normal rats,indicating that MSG-OB rats was established successfully.The long-term administration of high and middle dose of rhCNTF resulted in a significant and dose-dependent reduction of Lee's index,fat index,serum triglyceride(TG) and tatal cholesterol(TC),and to profoundly increased high density lipoprotein-cholesterol(HDL-C) in comparison with obese saline-treated control.It also reduced a significantly the level of serum glucose hypoglycemic effect.Conclusion rhCNTF has significant and dose-dependen antilipemic effects in MSG-rats.Along with the antilipemic effect,rhCNTF also produces a significant hypoglycemic effect.

Key words: Recombinant human ciliary neurotrophic factor(rhCNTF), MSG-rats, Antilipemic effect, Hypoglycemic effect