关键词: 高脂饲料, 酒精性肝损伤, 大鼠模型

Abstract: Objective To establish an ideal method to develop alcohol-induced liver injury in rats.Method Thirty healthy male Sprague-Dawley(SD) rats were randomly divided into two groups,control group(give NS) and model group(drinking red Star falls and feeding high fat diet),and 15 per group.All the rats were kept on the treatments for 4 weeks.Blood was collected from the orbital venous plexus at the end of experiment,and serum levels of ALT,AST,TC,TG,HDL-C,SOD,MDA,and hepatic glycogen content,as well as liver coefficient were detected. Results The liver coefficient and serum levels of TC,TG and HDL-C,hepatic glycogen,MDA,and SOD in model group increased significantly than those in control(P<0.05);Serum levels of ALT and AST in model group also increased significantly than those in control(P<0.01).Conclusion It is a successful method to develop the alcohol-induced liver injury in rats combining alcohol-drinking with high-fat diet feeding.

Key words: High fat diet, Liver damage, Rat model