硫薰山药对大鼠肝肾代谢功能的影响

实验动物科学

硫薰山药对大鼠肝肾代谢功能的影响

郭婕;周景洋;颜燕;赵海霞;程东;姚文环;

山东省疾病控制中心;山东中医药大学附属医院;

收稿日期:2010-04-28 修回日期:2010-04-28 出版日期:2010-04-28 发布日期:2010-04-28

Effect of Sulfur Fumigated Dioscorea opposita Thunb on Oxidative Stress in Liver and Kidney of Rats

GUO Jie1,ZHOU Jing-yang1,YAN Yan1,ZHAO Hai-xia2,CHENG Dong1,YAO Wen-huan1

(1.Shandong Center for Disease Control and Prevention,Jinan 250014,China)(2.Affiliated Hospital of Shandong University of Chinese Medicine,Jinan 250011,China

Received:2010-04-28 Revised:2010-04-28 Online:2010-04-28 Published:2010-04-28

摘要/Abstract

摘要： 目的观察硫磺熏蒸山药对雌、雄大鼠肝肾脂质过氧化水平和抗氧化能力的变化。方法将实验动物随机分为4组,其中3组分别给予山药饮片、市售熏蒸山药饮片、饱和熏蒸山药饮片水提浓缩液(5 g/mL),掺入基础饲料50 g/kg;正常对照组给予基础饲料。测定14 w后大鼠肝肾中丙二醛(MDA)含量、GSH-Px活力和SOD活力。结果(1)山药有一定抗氧化作用;(2)不同浓度硫磺薰蒸山药对不同脏器引起的氧化应激作用不同(与浓度有关),并且有器官差异性;⑶高浓度硫磺薰蒸山药对雌雄大鼠造成的氧化损伤作用,在不同脏器间存在性别差异。结论长期食用经高浓度硫磺熏蒸的山药有可能引起肝肾氧化损伤。

关键词: 山药, 硫磺熏蒸, 肝脏, 肾脏, 脂质过氧化作用

Abstract: Objective To investigate the influence of sulfur fumigated Dioscorea opposita Thunb on peroxidation and antioxidation in liver and kidney of rats.Methods Wistar rats were divided randomly into 4 groups,one control and three experimental groups administrated diets mixed with concentrated extractions from unfumigated,commercial and sulfur fumigated Dioscorea opposita Thunb respectively.The level of MDA and activities of SOD and GSH-Px 14 weeks after treatment were measured.Results(1) Dioscorea opposita Thunb enhanced the activity of antioxidation;(2) Level of oxidative stress is different in liver and kidney,and depended on the concentration of sulfur fumigated Dioscorea opposita Thunb extractions.(3) There were sexual differences in levels of oxidation damage by Sulfur fumigated Dioscorea opposita Thunb,so did in various organs.Conclusion It is possible to cause lipid peroxidation in liver and kidmey by long-term use of Sulfur fumigated Dioscorea opposita Thunb.

Key words: Dioscorea opposita Thunb, Sulphur fumigation, Liver, Kidney, Lipid peroxidation

郭婕;周景洋;颜燕;赵海霞;程东;姚文环;. 硫薰山药对大鼠肝肾代谢功能的影响[J]. 实验动物科学.

GUO Jie1,ZHOU Jing-yang1,YAN Yan1,ZHAO Hai-xia2,CHENG Dong1,YAO Wen-huan1. Effect of Sulfur Fumigated Dioscorea opposita Thunb on Oxidative Stress in Liver and Kidney of Rats[J]. laboratory animal science.

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