BALB / c 小鼠, SNP 分子标记, 生化标记, 遗传检测

The similarities and differences between the current national standard biochemical marker method and the Sanger sequencing detection mouse SNP molecular marker method in the evaluation of genetic quality of BALB / c mice were compared and analyzed, so as to provide a reference for the genetic quality control of inbred mice. Methods The genetic quality of the same batch of BALB / C mice was tested using 14 conventional biochemical marker loci and 34 common genetic analysis loci of SNP.The result evaluation, variation detection, chromosome coverage, animal welfare, operation  convenience and method repeatability were compared between the two methods. Results Six BALB / cmice with No. 2, 5 and 6 variants were detected by both methods, but only homozygous variants at 4 loci were detected by biochemical marker, while homozygous variants at 9 loci and heterozygous variants at 3 loci were detected by SNP molecular marker. Further comparison showed that SNP marker method was superior to biochemical marker method in chromosome coverage, animal welfare, operation convenience and method repeatability. Conclusion SNP marker method has more advantages in genetic quality control evaluation of inbred mice than the current national standard biochemical marker detection method, which can be used as a standardized genetic quality control technology for inbred experimental animals.

BALB / c mice, SNP molecular marker, biochemical markers, genetic monitoring