关键词: 免疫缺陷小鼠, 遗传质量, 基因型鉴定, PCR 检测方法, 自发突变基因

Abstract: Objective To establish three genotyping methods for immunodeficient mice. Method According to the genotype identification sequences of three immunodeficient mice, B6. Cg-Foxn1 nu / J, B6. Cg-Prkdc scid / SzJ, and C57BL / 6J-Lyst bg-J / J, provided on The Jackson Laboratory website, primers were designed and PCR reaction programs were optimized to establish PCR detection methods for corresponding spontaneously mutated genes. I n addition, the above corresponding methods were applied to genotype identification of BALB / cAnSlacNifdc-nu, SCID / Nifdc, and C57BL / 6JNifdc-bg mice preserved in the National Rodent Laboratory Animal Resources Center. Result Three simple methods were established using PCR combined with Sanger sequencing to detect spontaneous mutations in Foxn1 nu , Prkdc scid , and Lyst bg-J genes. Furthermore, the corresponding method were used to detect mutation sites in the genomes of three inbred mice strains, including BALB / cAnSlacNifdc-nu, SCID / Nifdc, and C57BL / 6JNifdc-bg, which were preserved in the National Rodent Laboratory Animal Resources Center. The genotyping results of the three mice strains were consistent with the corresponding information published on The Jackson Laboratory website. Conclusion The simple method for genotype identification of immunodeficient mice established in this study are beneficial for controlling and monitoring the genetic quality of immunodeficient mice. 

Key words: immunodeficient mice, genetic quality, genotype identification, PCR detection methods, spontaneously mutated genes