关键词: 呼吸运动, 膈神经放电, 膈肌放电, 同步记录

Abstract: Objective This study aimed to improve the experimental setup for investigating factors influencing respiratory motor regulation. The objective was to achieve real-time synchronous recording of phrenic nerve discharge, diaphragmatic muscle discharge, and respiratory movement in rabbits, and to calculate the time difference between phrenic nerve discharge and diaphragmatic muscle discharge (ΔT1) , as well as the time difference between diaphragmatic muscle discharge and the start of the respiratory curve ( ΔT2 ) . Method The phrenic nerve and diaphragmatic muscle of rabbits were isolated, and tracheal intubation was performed. The BL-420I information-integrated signal acquisition and processing system was used to simultaneously record the curves of phrenic nerve discharge, diaphragmatic muscle discharge, and respiratory movements under normal physiological state and different interventions ( increasing the null lumen, increasing the concentration of CO2 , intravenous injection of lactic acid solution at the ear margin, cutting the vagus nerve and stimulating the vagus nerve at the central end) . ΔT1 and ΔT2 were calculated, and 30 respiratory cycles were statistically analyzed. Result The synchronization curves of phrenic nerve discharge, diaphragmatic discharge and respiratory movement were recorded simultaneously under normal physiological state and various intervention conditions by applying BL-420I. Under normal physiological state, ΔT1 = ( 32. 95±5. 34) ms, ΔT2 = ( 0. 366±0. 028) s; after increasing the null lumen, ΔT1 = (28. 63±5. 72)ms, ΔT2 = (0. 295±0. 020) s; after inhalation of CO2 , ΔT1 = ( 20. 72 ± 5. 51) ms,ΔT2 = ( 0. 250 ± 0. 016 ) s; After intravenous injection of lactate, the ΔT1 = ( 27. 76 ± 8. 90) ms, ΔT2 = (0. 317± 0. 013 ) s; compared with the normal conditions, all three intervention conditions showed significant difference ( P < 0. 01 ) , indicating statistical significance. Conclusion The method of real-time synchronous recording of phrenic nerve discharge, diaphragm discharge and respiratory movement in rabbits was successfully established, and the calculation method for ΔT was determined, which is beneficial for students’ better understanding of the regulatory role of the respiratory center in respiratory movements. Objective This study aimed to improve the experimental setup for investigating factors influencing respiratory motor regulation. The objective was to achieve real-time synchronous recording of phrenic nerve discharge, diaphragmatic muscle discharge, and respiratory movement in rabbits, and to calculate the time difference between phrenic nerve discharge and diaphragmatic muscle discharge (ΔT1) , as well as the time difference between diaphragmatic muscle discharge and the start of the respiratory curve ( ΔT2 ) . Method The phrenic nerve and diaphragmatic muscle of rabbits were isolated, and tracheal intubation was performed. The BL-420I information-integrated signal acquisition and processing system was used to simultaneously record the curves of phrenic nerve discharge, diaphragmatic muscle discharge, and respiratory movements under normal physiological state and different interventions ( increasing the null lumen, increasing the concentration of CO2 , intravenous injection of lactic acid solution at the ear margin, cutting the vagus nerve and stimulating the vagus nerve at the central end) . ΔT1 and ΔT2 were calculated, and 30 respiratory cycles were statistically analyzed. Result The synchronization curves of phrenic nerve discharge, diaphragmatic discharge and respiratory movement were recorded simultaneously under normal physiological state and various intervention conditions by applying BL-420I. Under normal physiological state, ΔT1 = ( 32. 95±5. 34) ms, ΔT2 = ( 0. 366±0. 028) s; after increasing the null lumen, ΔT1 = (28. 63±5. 72)ms, ΔT2 = (0. 295±0. 020) s; after inhalation of CO2 , ΔT1 = ( 20. 72 ± 5. 51) ms,ΔT2 = ( 0. 250 ± 0. 016 ) s; After intravenous injection of lactate, the ΔT1 = ( 27. 76 ± 8. 90) ms, ΔT2 = (0. 317± 0. 013 ) s; compared with the normal conditions, all three intervention conditions showed significant difference ( P < 0. 01 ) , indicating statistical significance. Conclusion The method of real-time synchronous recording of phrenic nerve discharge, diaphragm discharge and respiratory movement in rabbits was successfully established, and the calculation method for ΔT was determined, which is beneficial for students’ better understanding of the regulatory role of the respiratory center in respiratory movements. 

Key words: respiratory movements, phrenic nerve discharge, diaphragm discharge, synchronized recording