关键词: 麻醉, 免疫缺陷小鼠, 速眠新Ⅱ, 舒泰 50 配比

Abstract: Objective To explore the anesthesia effect of the new uncontrolled anesthetic drug Zoletil 50 and Sumianxin Ⅱ on SPF mice, hoping to find out a method that can achieve the ideal anesthesia effect for all strains of mice. Method Referring to the instructions of the drug, taking the dosage of dogs, cats and rabbits as reference, the mice of each strain were administered by intraperitoneal injection by using Sumianxin 11 ( 30-fold dilution) alone; Zoletil 50 ( 10-fold dilution) alone; mixed Sumianxin 11 and Sutai 50 both diluted by 10 times with 1 ∶ 1 mixing ratio; and mixed Sumianxin 11 ( 20 times dilution) and Zoletil 50 ( 5 times dilution ) 1 ∶ 1 mixing ratio, erspectively, and the administration dose was 0. 1 mL / 20 g. Five CD-1 strains were used to administer the single drug, and six strains were used to administer the mixed drug, with 10 mice in each group. Result All the mice with Sumianxin 11 died, and the mice with Zoletil 50 alone could not reach the surgical requirement of anesthesia. In both groups of the mixed Sumianxin 11 and Sutai 50, except for one mouse that died due to improper insulation, the rest of the mice could reach the requirement of surgical anesthesia and wake up normally. Conclusion Converting the result to the working concentration, SuMianxin Ⅱ with the concentration of 10-25 mg / kg combined with the concentration of 10 - 25 mg / kg of Sutai 50 at a ratio of 1 ∶ 1 can achieve the ideal effect in the actual use of intraperitoneal injection anesthesia in mice, suggesting a strong application value in mouse experimental anesthesia.

Key words: anesthesia, immune deficiency mice, Somianxin Ⅱ, Zoletil 50 ratio