关键词: 实验动物, 猴痘病毒, 荧光 PCR

Abstract: Objective To investigate monkeypox virus infection in lab animals in Beijing. Method Refer to the guidance of the World Health Organization ( WHO) for laboratory testing of monkeypox virus, a total of 642 animals in Beijing were detected by fluorescent PCR, including 127 monkeys, 215 mice, 80 rats, 40 guinea pigs, 10 hamsters, 85 rabbits and 85 dogs, Pharyngeal swabs were collected from monkeys, cecum was collected from rats, guinea pigs and hamsters, and anal swabs were collected from rabbits and dogs. The collected samples were added with appropriate amount of sterile PBS, The cecum samples were fully homogenized, the swab samples were swirled for 1 min, repeatedly freezethawed at -70 ℃ for 3 times, the supernatant was centrifuged to extract nucleic acid, and the monkeypox virus DNA was detected by fluorescent PCR, and the result were analyzed finally. Result 127 monkeys, 215 mice, 80 rats, 40 guinea pigs, 10 ground mice, 85 rabbits and 85 dogs were all negative for monkeypox virus DNA by fluorescent PCR. Conclusion Monkeypox virus infection has not been found in lab animals in Beijing at present.

Key words: lab animal, monkeypox virus, fluorescent PCR