关键词: 关键词:冠脉微循环障碍, X , 射线衍射增强成像, 血管铸型, 双参宁心胶囊, 三维可视化

Abstract: Abstract: Objective To explore the three-dimensional visualization method of coronary microvessels, and whether Shuangshen Ningxin capsule improves the three-dimensional morphology of coronary microvessels in rats with coronary microvascular dysfunction ( CMD) . Method Male healthy SD rats were randomly divided into 3 groups, the sham operation group, the model group and the Shuangshen Ningxin capsule group. Shuangshen Ningxin capsule ( 90 mg / kg ) group animals were given intragastric administration for 7 consecutive days. The sham operation group and the model group were given an equal volume of normal saline gavage. Seven days later, the coronary microcirculation dysfunction model was established, and the specimens were sacrificed 24 hours after the operation. Coronary vascular casting and image acquisition was conducted. The X-ray diffraction enhanced images were obtained, the tomographic images were used for the three-dimensional reconstruction, 3D model was built to observe the distribution of coronary microvessels. Result Using self-curing dental tray materials can quickly make rat coronary vascular cast specimens, the smallest resolvable blood vessel diameter is about 40 μm. A threedimensional model of the coronary microvascular network is obtained after the three-dimensional reconstruction of the X-ray diffraction enhanced image, the smallest resolvable blood vessel can reach 10 μm. Both vascular casting and X-ray diffraction enhanced imaging reflect the changes in the spatial distribution of coronary microvessels in CMD rats, also the intervention of Shuangshen Ningxin capsule can also significantly improve the coronary microvasculature of animal models. Conclusion The X-ray diffraction enhanced imaging method can construct a three-dimensional visualization model of the coronary microvascular network to a certain extent, can reflect the abnormality of coronary microvascular in rats with coronary microvascular dysfunction, the effect of Shuangshen Ningxin capsule in increasing coronary microvessel density can also be reflected by this method.

Key words: Key words:coronary microvascular dysfunction, X-ray diffraction enhanced imaging, vascular cast, Shuangshen Ningxin capsule, 3D visualization