关键词: 结肠炎, 柚皮苷, 肠道菌群

Abstract: Objective To explore the effect of naringin ( NG) on the intestinal flora of mice with DSS-induced colitis. Method C57BL / 6 male mice were used to construct animal models. Fifteen mice were divided into the normal group, the model group and the NG group, each group with 5 mice. Except the normal group, the other 2 groups were given 4% ( m / v) DSS as freely drunk, and the NG group was given NG for intervention from the second day. The mice were sacrificed on the 9th day, and their colon and feces were collected. Western blot was used to detect the inflammatory molecule protein p-NF-κB-p65 and the apoptosis protein B lymphama 2 ( Bcl-2) . Using PCR-denaturing gradient gel electrophoresis ( PCR-DGGE ) technology to analyze the structure of mouse intestinal flora. Result The expressions of p-NF-κB-p65 protein and Bcl-2 protein in the model group were significantly different from those in the normal group and the NG group had different degrees of changes after NG intervention. The bands in PCR-DGGE were performed by unweighted pairwise arithmetic, the UPGMA algorithm was used for cluster analysis, and the result showed that the structure of the colonic flora of mice was significantly different in each group. Conclusion NG can effectively improve the symptoms of DSS-induced colitis and improve the intestinal flora of mice.

Key words: colitis, naringin, intestinal flora