Abstract: Abstract: Objective To design an experimental animal atomization box with a household medical atomizer and facilitate airway disease research in experimental animals. Method A household atomizer and a separate ventilating squirrel cage were combined and used to transform into an experimental animal atomization box. We built a mouse asthma model with the self-designed atomization box and ovalbumin. Result The atomization box could produce enough fog for 1 - 20 mice experiment rapidly. We successfully built the mouse asthma model with the atomization box. Conclusion We applied the atomization box to build the mouse allergic asthma model and observed significant lung tissue inflammation. This method provides operational guide for experimental animal inhalation experiment with convenience and low cost.