关键词: 过敏性鼻炎, 特异性脱敏法, 柳树花粉, 免疫治疗

Abstract: Objective Establish mouse models of willow pollen allergic rhinitis and immune tolerance treatment of the mouse models. Method Pollen protein was extracted and mouse models of allergic rhinitis was established by intraperitoneal injection of pollen protein and nasal stimulation. By subcutaneous injection of increasing dosage of allergen into mice, the behavioral symptoms of mice were observed, the morphological changes of nasal mucosa were compared, and the content of specific sIgE in serum was detected, so as to study the therapeutic effect of specific desensitization therapy on allergic rhinitis. Result Before treatment, allergic symptoms such as scratching, sneezing and runny nose appeared in mice. Eosinophilsin nasal mucosa were significantly more than those in control group, and the content of specific sIgE in serum was higher than that before stimulation. These results indicate that the allergic rhinitis mouse models induced by willow pollen has been successfully established. After treatment, allergic symptoms of mice gradually decreased to disappear, eosinophils in nasal mucosa gradually decreased, and specific sIgE content in serum decreased. Conclusion Willow pollen can establish allergic rhinitismodelsin mice, and specific desensitization method could treat allergic rhinitis in mice.

Key words: Allergic rhinitis, Specific desensitization method, Willow trees pollen, immunotherapy