关键词: 犬肾细胞系, 干扰素受体, 转录表达水平

Abstract: Objective To understand the expression of IFNs and its receptor molecules in Madin-Darby Canine Kidney (MDCK) cell line infected with canine adenovirus type 1 ( CAV-1 ) . Method Several SYBR real-time quantitative PCR( RT-PCR) method were established to detect IFN-β, IFN-βreceptor. ( IFNAR1 ) IFN-λ1, IFN-λ1 receptor( IFN-λ1R) , IFN-λ3 and IFN-λ3 receptor( IFN-λ3R) in canine. The relative transcriptional levels of IFNs and its receptor molecules in Poly I: C treated-MDCK cells and CAV-1 treated-MDCK cells were analyzed. Result The transcriptional level of IFN-λ3R were significantly higher than that of other IFNs and receptor molecules in normal MDCK cells. The transcriptional levels of IFN-λ1R, IFN-λ3 , IFN-λ3R increased significantly in MDCK cells treated with Poly I: C for 24 h, while those of IFN-β, IFN-λ1, and IFNAR1 remained unchanged. However, the transcriptional level of IFN-α was the most highly than others in MDCK cells infected with CAV-1 for 24 h. Conclusion The result showed that there are different levels of various IFN molecules and their receptor transcripts in MDCK cells, and the express pattern varies after induction by. Poly I ：C or adenovirus.

Key words: Madin-Darby Canine Kidney cells, Interferon receptors, transcriptional expression levels