关键词: 破伤风疾病模型, 行为变化, 组织外观及组织病理比较

Abstract: Objective The aim of this paper is to establish the model of tetanus and complete the research of Clostridium tetani. Method ICR mice were used as the experimental animal model, and tetanospasmin ( TeNT ) was injected intraperitoneally. The behavior change and tissue (lung, liver and brain ) comparison of each group were observed in this experiment. Result Tetanospasmin can cause the body spasm , limbs rigidity spasmodic and opisthotonus , also cause liver to appear cell denaturation , cerebrum neuron necrosis , cause mice to die. However , when the dose of Tetanospasmin is lower than 10 4 test dose, it will not cause death in mice, and there is no obvious pathological change. Conclusion The model of tetanus disease in ICR mice was established.

Key words: model of tetanus, behavior changes, comparison of tissue