关键词: 小鼠细小病毒(MMV), 检测能力验证, 酶联免疫吸附实验

Abstract: Objective Through the capability verification of Minute virus of mice (MMV ) antibody in laboratory mice detection, to investigate the capacity of experimental animal quality control laboratories, so as to promote the improvement of laboratory animal quality testing level. Method According to the capability verification scheme approved by CNAS, the serums were calibrated, and passed the stability and homogeneity test. Then the serums were used as the proficiency testing samples. The samples were randomly numbered, and then sent to the units participating in the capacity verification, and attach the work instruction. This capability verification was a qualitative test, and the test method was ELISA. The participating units are required to submit the test report and copy of original record within the prescribed time limit, The satisfactory result is that the test result of the sample is consistent with the pre-test result, the unsatisfactory result is that the sample test result is inconsistent with the pre- test result or the participating units did not feedback the result. The participants must submit the test reports and original records in time. The feedback result were judged by the rate of concordance with the anticipated result. Result The result of uniformity test and stability test of samples all met the requirements of capability verification for samples. There were 33 animal testing institutions from 19 provinces and cities took part in this capability verification. The results ubmitted by 33 testing institutions are all satisfactory, and the satisfaction of this capability verification is 100%. Conclusion It can be reflected the testing ability and level of China ‘s testing laboratories for the quality control of laboratory animal by this capability verification plan , and the ability for detection of minute virus of mice antibody in laboratory mice was high .

Key words: Murine minute virus , MMV, Detection capability verification , ELISA