关键词: 安乐死, 二氧化碳, 大鼠, 小鼠

Abstract: Rats and mice are currently the most widely used laboratory animals,and their euthanasia method have been heatedly debated.The carbon dioxide euthanasia method has the advantages of simple use,low risk and low cost.In order to make the implementation of carbon dioxide euthanasia more in line with the requirements of experimental animal welfare ethics,the application of this technology should fully consider the pressure,pain and other reactions of experimental animals.This paper reviews the evaluation system of euthanasia by inhaling carbon dioxide,the combined use of carbon dioxide and other anesthetics,and the specific implementation method of carbon dioxide euthanasia in mice and rats.The purpose of this review is to provide useful technical references to caorbon dioxide for euthanasia to mice and rats

Key words: Euthanasia, CO2, Rat, Mice