关键词: I型干扰素受体, 基因敲除, 小鼠, 繁育, 基因型鉴定

Abstract: Objective To analyze the identification and breeding method of ifnar knockout mice, and provide an ideal animal model for virus further studying. Method The introduced homozygous ifnar gene knockout mice were bred and reproduced in one cage with one male and two female mice together. The offspring mice genomic DNA was extracted from mice tail and amplified by PCR, and PCR products were analyzed by agarose gel electrophoresis for genotype identification. Result The homozygous ifnar knockout mice were breeding successfully in our facility,and the genotype can be identified by our established PCR method .

Key words: Ifnar, knockout, mouse, breeding, genotype identification