关键词: 绿色荧光裸鼠, 雌激素, 孕激素, 泌乳素, 泌乳素受体

Abstract: To understand the changes in plasma levels of estrogen, progesterone, prolactin, and prolactin receptor mRNA in mice during pregnancy and lactation. Plasma levels of progesterone were measured by electro-chemiluminescence and relative mRNA levels of prolactin and prolactin receptor were measured by fluorescence quantitative PCR. During the gestation period, estrogen and prolactin levels decreased continuously, progesterone levels increased first and then decreased slowly, prolactin mRNA levels were low in tissues, and prolactin receptor levels were high.During lactation, the levels of estrogen and progesterone are relatively stable, the levels of prolactin mRNA in tissues are relatively high, and the levels of prolactin receptors are relatively low. Different levels of sex hormones in nude mice with green fluorescence were different in different periods, and they participated in the process of pregnancy regulation in nude mice. It provides basic reference value and endocrine level for solving the problems of female mouse infertility and abortion.

Key words: Green fluorescent nude mice, Estrogen, Progesterone, Prolactin, Prolactin receptor