关键词: 小鼠, 异丙肾上腺素, 慢性心肌缺血, 评价标准

Abstract: Objective To prepare chronic myocardial ischemia models in mice and determine the best molding dose, and to discuss the evaluation indexes of the model which could provide reference for the preparation of related models and the evaluation of their efficacy. Method Three model groups of different doses were treated with subcutaneous injection of isoproterenol (32 mg/kg, 16 mg/kg, 8 mg/kg) for three consecutive days, after 24 hours, 8 days and 15 days ECG were recorded. On the eighth day and the fifteenth day, markers of myocardial injury in the blood were detected and the morphological changes of myocardium were observed. Result The ECG, markers of myocardial injury and pathology were all markedly changed in the group of 32 mg/kg isoproterenol(P<0.05 or P<0.01), and the mortality rate was in the acceptable range. The result could be used for evaluating the efficacy of the medicine for long-term treatment of chronic myocardial ischemia.The mortality rate of the group of 16 mg/kg isoproterenol was low, and indexes of ECG and markers of myocardial injury were better than that of the group of 32 mg/kg isoproterenol, and the dose did not cause irreversible pathological damage to the heart muscle. Conclusion The result showed that the chronic myocardial ischemia models in mice can be established by using two different molding dose according to different test expectation.

Key words: mice, chronic myocardial ischemia, isoproterenol, evaluation criterion