关键词: 坏死性小肠结肠炎, 模型建立, 新生儿, 大鼠, 脂多糖

Abstract: Objective The present study aimed to modify and evaluate necrotizing enterocolitis (NEC) models widely used in rats, and to determine the better method . Method Ninety rat pups were divided into five groups: Group A as control (n=10). Group B to E (n=20, respectively), rats were raised with simulated milk and subjected to hypoxia, cold stress, LPS with different ways and doses (Group B p.o. LPS 5 mg/kg; Group C p.o.LPS 10 mg/kg; Group D i.p. LPS 2.0 mg/kg; Group E i.p. LPS 5 mg/kg) per 12hours for 72hours. Followed by observing and recording body weight, survival time and sample collection, gross examination, HE staining, NEC injury scored, TNF-α levels in the intestine and kidneys were detected. Result We found that Group A rats were normal, but rats from groups B-E exhibited NEC clinical characteristics, but to varying degrees in different groups. The Group D (i.p.LPS 2.0 mg/kg) has comparatively lower mortality rate, no- NEC-associated death rate, higher morbidity rate and moderate TNF-α level in intestine and kidneys and NEC injury score when compared to other groups. Conclusion The modified method that through combination of formula feeding, hypoxia, cold stress, and low-dose LPS intraperitoneal injection is an optimal strategy of modeling rat pup NEC.

Key words: Necrotizing Enterocolitis, modeling, neonates, rat, Lipopolysaccharide