关键词: <, p>, 肾阳虚, 小鼠, 动物模型, 氢化可的松<, /p>

Abstract: Abstract: To compare the advantages and disadvantages between different dosage and methods of administration of cortisone in deficiency of Kidney Yang animal models，we used intragastric administration，intraperitoneal injection and subcutaneous injection ways to mice with different doses ( 12. 5，25，50 mg / kg) of hydro-cortisone for 9 days to investigate the variations on mortality rate，general state，weight，anus temperature，autonomic activities，swimming duration and viscera index． After 9 days，general status in mice is poorer，the 25，50 mg / kg dose group are more obvious． Besides subcutaneous injection of 25 mg / kg group，other groups weight was significantly lower than before building; besides intragastric and intraperitoneal injection of 12. 5 mg / kg，subcutaneous injections of 25 mg / kg group，number of autonomic activities in mice decreased to some extent; in addition to the various ways of 12. 5 mg / kg group，the swimming time was significantly shortened than the blank group; intraperitoneal injection and subcutaneous injection of 25 mg / kg group spleen index，all ways of 12. 5 mg / kg group of adrenal gland index compared with the blank group has no obvious change，the rest of the group of spleen，thymus and adrenal gland index were significantly reduced，seminal vesicle and preputial glands of each dose group have no obvious change． So we synthesized the inspection indexes，intragastric administration 25 mg / kg dose group is superior to that of other groups． All the indexes can show the signs and symptoms of Kidney Yang Deficiency，it is more reliable Kidney Yang Deficiency model method．

Key words: <p>Kidney Yang Deficiency, mice, animal model, hydro cortisone</p>