关键词: <, p>, 小鼠腺病毒, 荧光定量 PCＲ<, /p>

Abstract: Abstract: Objective Establish a specific qPCＲ detectionmethod based on TaqMan probe to investigate mouse adenovirus ( MAdV) infection in gerbils，mice and other laboratory animals． Method Genomic sequences of mouse adenoviruses ( MAdV-A and MAdV-3) were downloaded and primers and probes were designed at the best conservative genes which were selected after comparative analysis． Screened and tested the specificity，sensibility， repeatability and stability of the best primers and probe，utilized this q-PCＲ method to detect 41 samples of gerbils and voles． Ｒesult MAdV-3 was chosen as the best from three pairs of primers and probes，which could distinguish MAdV，SAdV，FAV and TAV and other 24 viruses，the minimum detectable amount of MAdV plasmid DNA template is 4. 5copies / reaction; the minimum detectable amount of MAdV pure virus and virus /tissue simulate sample DNA template both are 440copies / reaction． Conclusion The TaqMan qPCＲ detection method established in this manuscript has good specificity，sensitivity，repeatability and stability，which might be used to detect MAdV in laboratory animals and relevant biological products．

Key words: <p>Mouse adenovirus( MAdV), Fluorescence quantitative PCＲ</p>