关键词: <, p>, syncytinA, 条件敲除小鼠, 胚胎显微注射<, /p>

Abstract: Abstract: Objective To establish syncytinA conditional knock out mice to investigate the function of syncytinA in the placental development and preeclampsia pathology． Method After successful incorporation of loxp synA exon by homologous recombination in ES cells，we used CRISPR-Cas9 technology to construct the syncytinA-loxp mice．We construct syncytinA-loxp transgenic vector and sgRNA，they were injected with Cas9 into fertilized eggs of C57 by prokaryotic microinjection methods，and these eggs were transplanted into pseudopregnant mice． The genotype of transgenic mice was identified by PCR． Then loxp positive mice were mated with WT ( wild type) to get syncytinAloxp mice． In order to detect whether syncytinA knockout． SyncytinA－ / － was obtained by mating with prime1cre and zp3cre． SyncytinA － / － was identified by PCR and q-PCR． Conclusion SyncytinA conditions knockout mouse was successfully constructed in present study．

Key words: <p>syncytinA, conditional knock out mouse, embryo microinjection</p>