关键词: 拉布拉多犬, 神经类型, 单核苷酸多态性( SNP), 分子遗传学诊断

Abstract: Abstract: Objective Analysis of 6 SNPs associated to nerve pattern related 4 genes in Labrador Ｒetrievers to provide a early-stage molecular genetic index for nerve pattern diagnostics． Method According to the behavior evaluation standards of China guide dog training center，the dogs belong to typical nerve patterns ( quiet，active， and timid ( weak ) type，4 dogs each) were selected． The vein blood was sampled and the genomic DNA was isolated from blood cells． The PCＲ/sequencing and PCＲ-ＲFLP method were used to analyze the variants of 6 SNPs in nerve pattern related 4 genes: ( COMT ( G482A) ，MOAB ( T199C) ，ABCB1 ( A985T，T3442C，377 － 378 insC) ，GNB1L( 961 － 962 insG) ) ． Furthmore，the association between the SNP variants and nerve patterns was analyzed by Chi-square Test． Ｒesult G/A variants in COMT ( G482A) SNP locus in the proportion of timid dogs was significantly higher than that in the active and quiet dogs ( P = 0. 014; P = 0. 014) ，A/A variants in COMT ( G482A) SNP locus in the proportion of active dogs was significantly higher than that in the quiet and timid dogs ( P = 0. 025; P = 0. 025 ) ; T /C variants in MAOB ( T199C) SNP locus in the proportion of quiet dogs was significantly higher than that in the active and timid dogs ( P = 0. 025; P = 0. 025 ) ; T /A variants in ABCB1 ( A985T) SNP locus in the proportion of active dogs was significantly lower than that in the quiet and timid dogs ( P = 0. 014; P = 0. 014 ) ; T /C variants in ABCB1 ( T3442C) SNP locus in the proportion of timid dogs was significantly lower than that in the quiet and active dogs ( P = 0. 025; P = 0. 025) ; No ABCB1 ( 377 － 378 insC) and GNB1L ( 961 － 962 insG) SNP loci was found． Conclusion G/A and A/A variants in COMT ( G482A) 、T /C variants in MAOB ( T199C) 、T /A variants in ABCB1 ( A985T) and T /C variants in ABCB1 ( T3442C) are significantly associated to nerve patterns and might be used as a molecular genetic index for nerve pattern diagnostics in Labrador Ｒetrievers． However，further large sample analysis is required．

Key words: Labrador Ｒetrievers, nerve pattern, SNP, molecular genetic diagnostics