关键词: 基因敲除小鼠, HO-1, 纯合子, 杂合子

Abstract: Abstract: Objective To breed and identify HO-1 gene knock-out mice． Method The HO-1 gene knock-out heterozygote mice were bred in specific pathogen free environment and copulated with wild genotype mice． The Genomic DNA of the off-springs was extracted and the HO-1 gene was specifically amplified by polymerase chain reaction． Using dideoxy-sequencing method the genotypes of off-springs present as three types: wild genotype， heterozygote genotype and homozygote genotype． Heterozygote genotype mice were copulated for further breeding， while the homozygote genotype mice were used for research． Ｒesult Breeding and reproducing were both successful，and we obtained heterozygote genotype and homozygote genotype mice with HO-1 gene knockout． Conclusion Correct methods of breeding，reproducing and identifying can effectively obtain HO-1 gene knock-out homozygote mice from heterozygote mice．

Key words: knock-out mice, heme oxygenase-1, homozygote, heterozygote