关键词: 大鼠, 脊髓损伤模型, 双侧半横切

Abstract: Abstract:Objective To establish a novel rat spinal cord injury model so as to furnish a suitable animal model for clinical research of bilateral spinal cord injury at different planes． Method Forty-five Sprague-Dawley rats were randomly divided into bilateral spinal cord hemi-section group ( group A，n = 15)，spinal cord transection group (group B，n = 15 ) and sham-operation group ( group C，n = 15 )． After operation，the activities，ingesta， excretion，wound infection，death and other complications of rats in different groups were observed． Besides，BBB locomotive function scores and inclined plane test were performed to evaluate rats’behavioral function． Result After operation，the activities and ingesta were decreased in group A． Meanwhile，53. 33% of rats suffered from the uroschesis and 40. 00% died in the course of 28 － days’evaluation． In group B，The activities and injesta were obviously decreased，66. 67% of rats suffered from the uroschesis and 60. 00% died in 4 weeks post-injury． In group C，whereas，rats’activities and ingsta did not decrease as well as no complications and death occurred． The mean scores of BBB and maximum angle of inclined plane in group A and B were significantly lower than that in group C (P ＜ 0. 05)，but these parameters had no remarkable difference between group A and group B． Conclusion The spinal cord injury model with bilateral hemi-sections at different levels possesses the distinctions of lower incidence of uroschesis and mortality，and has not significant difference compared with the traditional spinal cord trans-ection model in behavioral evaluation． Thus，it is a suitable model for clinical research on bilateral spinal cord injury at different levels．

Key words: rats, spinal cord injury model, bilateral hemi-sections