关键词: 负压, 梯度电泳凝胶, PCR, PCR-DGGE

Abstract: Abstract: Objective To compare gel solidification time of DGGE before and after the negative-pressure process and to explore if the change of the negative-pressure treatment of the experiment can affect the PCR-DGGE results． Method Using amplified PCR products from two different primers ，run the denaturing gradient gel electrophoresis of the two products before and after different negative-pressure treatment，respectively． Result Compared with the results without the negative-pressure treatment process，the experimental results with negative-pressure treatment were significantly different，but the denaturing gel solidification time is not significantly affected after the vacuum treatment． Conclusion Negative-pressure process has significant influence on PCR-DGGE results，and it is an indispensable part of the experiment．

Key words: negative-pressure, gradient electrophoresis gel, PCR, PCR-DGGE