关键词: 拉布拉多犬, 金毛猎犬, 毛色基因, 单核苷酸多态性

Abstract: Abstract: Objective To establish the method for detecting SNP loci of coat color gene MC1R R306ter ( c． C916T) and TYRP1 Q331ter ( c． C991T) in canine． Method In order to establish the SNP detecting method，the SNP of coat color gene MC1R c． C916T and TYRP1 c． C991T in canine were detected by using PCR / sequencing method． And then，using the established method，the genotypes of coat color genes in 19 Labrador and 15 Golden Retriever dogs were investigated． Result The established PCR / sequencing method could detect the SNP loci for TYRP1 c． C991T clearly and effectively． However，there was a specific peak in the PCR / sequencing results for MC1R gene c． C916T SNP loci． Using cloning / sequencing method，we confirmed that the specific peak in the PCR / sequencing results of MC1R gene was heterozygous SNP loci． The genotype analysis for color genes showed that Golden Retriever dogs were all homozygous eeBB ( n = 15) genotype，no other genotypes were detected． For Labrador， black dogs showed EeBB ( n = 6) and EeBb ( n = 2) genotypes，yellow dogs showed eeBB ( n = 9) and eeBb ( n = 2) genotypes． No others genotypes such as EEBB and EEBb ( black ) or eebb ( yellow ) were detected． Furthermore，because there was no chocolate Labrador dog，so no corresponding Eebb or EEbb genotypes were detected． Conclusion The PCR / sequencing method for detecting the SNP loci of coat color gene MC1R R306ter ( c． C916T) and TYRP1 Q331ter ( c． C991T) in canine is successfully established． These works provide the basis for investigating the correlation between coat color genotypes and training success rates of guide dogs．

Key words: Labrador, Golden retriever, coat color gene, single nucleotide polymorphism ( SNP )