关键词: 15-KETE, 肺动脉环, 缺氧, 细胞外信号调节激酶, 肺动脉血管内皮细胞

Abstract: Abstract:Objective To observe the effects of EＲK1 /2 signaling pathway and vascular endothelium cells on 15- KETE-induced chronic hypoxic pulmonary artery constriction in rats using organ bath technique． Method Sixteen healthy Wistar rats (220 ± 20) g were randomly divided into two groups ( n = 8 each group) : normoxia and hypoxia groups housed in fresh air ( FiO2 = 21% ) and hypoxic air ( FiO2 = 10% ) in a hypoxic box，respectively． Pulmonary arteries( PA) were extracted after 9 days and cut into rings ( 0. 5 ～ 1. 0mm in diameter and 3mm in length) for organ bath experiments． The ring tensions before and after 15-KETE induction were compared． Influences of EＲK1 /2 upstream kinase inhibitor U0126 and endothelium integrity on 15-KETE induced HPV were investigated． Ｒesult With concentration increasing from 0 to 10 － 6 mol /L，15-KETE increased PA rings tension gradually in a dose-dependent fashion，and significantly constricted PA rings from the hypoxic rats． Ｒesponse of the hypoxic rings were significantly greater than that of normoxic ones (P ＜ 0. 05) ． The ring tensions induced by 15- KETE in endothelium-intact PAs were significantly greater than that in endothelium-denuded ones ( P ＜ 0. 05 ) ． U0126 significantly reduced vasoconstriction induced by 15-KETE in both endothelium-intact and -denuded rings． ( P ＜ 0. 05 in both) ． Conclusion 15-KETE significantly constricted PA rings in hypoxic rats in a dose-dependent fashion; EＲK1 /2 signaling pathway and vascular endothelium cells may be involved in vasoconstriction induced by 15-KETE，and EＲK l /2 in pulmonary artery smooth muscle cells may also be involved in vasoconstriction induced by 15-KETE in rats．

Key words: 15-KETE, pulmonary artery ring, hypoxia, EＲK1 /2, endothelium cells of pulmonary artery