关键词: 皮肤屏障, 胶带法, 丙酮乙醚法, 动物模型

Abstract: Abstract:Objective To evaluate two kinds of skin mechanical barrier disturbance model of mice based on the concentration of epidermal ceramides． ，thickness and tissue pathology． Method After the stratum cornea of mice were damaged using adhesive tape and mixture of acetone and ether respectively for five days，concentration of epidermal ceramides，thickness and pathology were examined． Ｒesult Concentration of epidermal ceramides and thickness in both model groups were lower than those in control． Pathological changes were much obvious in the adhesive tape treated group，although they were observed in both models． Conclusion Both of the two methods can cause skin mechanical barrier disturbance in mice． Skin mechanical barrier disturbance caused by adhesive tape was more reliable and easily to be achieved．

Key words: skin barrier, adhesive tape, mixture of acetone and ether, animal model