关键词: 维甲酸, 昆明小鼠, 腭裂模型

Abstract: Abstract:Objective To study when and how much to dose KM mice while creating the cleft palate model induced by ＲA ( retinoic acid) ． Method Divide randomly sixty KM mice which have been identified in pregnancy into four groups (A，B，C，and D)，there were fifteen ones in each group． The mice of group A，B，C were tube-fed ＲA( retinoic acid) with 20 mg / kg、50 mg / kg and 80 mg / kg respectively at the time of GD10( gestation day 10)， while the ones of group D tube-fed with 0. 20 mL corn oil at the same time． All the mice were allowed to reproduce naturally and then record the mortality of pregnant mice，stillbirth rate and incidence of cleft palate in the live fetuses． Data were compared between groups with χ2 test using SPSS16. 0 software package． Ｒesult The incidence of live fetuses with cleft palate in group B was 93. 59%，in group C which was 20. 00%，there was significant difference in the two groups (P ＜ 0. 007)，but the stillbirth rate and mortality of pregnant mice in group C were 40% and 76. 19%，which were higher than those in group B，the differences were statistically significant ( P ＜ 0. 007) ． In addition，the pregnant mice in remaining group A and D were without the occurrence of death and the fetal mice of these groups neither appear stillbirth nor cleft palate deformity． Conclusion Tube-feeding the KM mice with ＲA 50 mg / kg at GD 10 is an appropriate  method of setting up cleft palate model

Key words: ＲA, KM mice, cleft palate model