实验动物科学 ›› 2013, Vol. 30 ›› Issue (04): 44-45.

• 研究简报 • 上一篇    下一篇

大鼠多次采血常用的三种方法探讨

  

  1. ( 河南大学医学院细胞与分子免疫学重点实验室,开封475004)
  • 出版日期:2013-08-31 发布日期:2014-05-13
  • 基金资助:

    国家自然科学基金( No. 30972687)

Exploration on Three Common Methods of Multiple-drawblood in Rat

  • Online:2013-08-31 Published:2014-05-13

摘要: 摘要: 目的探讨大鼠在1 天内或者短时间内需要多次采血常用的三种方法,提高大鼠实验的成功率。方法采 用90 只雄性Wistar 大鼠,分别用眼眶后静脉采血法、颈静脉采血法及心脏穿刺采血法进行采血实验。结果眼眶 后静脉采血法: 采血0. 2—0. 3 mL/次,每只大鼠3—4 次/d。颈静脉采血法: 采血0. 2—0. 4 mL/次,每只大鼠6—8 次/d。心脏穿刺采血法: 采血0. 3—0. 4 mL,每只大鼠3—5 次/d。结论三种采血方法取血量相对比较大,血液标 本质量高,更利于大鼠的动物实验,但各有应注意的事项。

关键词: 大鼠, 采血方法, 多次

Abstract: Abstract: Objective To study the commonly used three kinds of method for rat venous blood sampling a day or in a short period of time. Method Total 90 male Wistar rats were used. Three methods were used for the blood sampling,angular vein blood sampling method,the jugular vein blood sampling method and the cardiac puncture blood sampling method. Result 0. 2—0. 3 mL blood can be collected from the angular vein everytime,and 3— 4 times a day; 0. 2—0. 4 mL blood can be collected from the jugular vein everytime,and 6—8 times a day; 0. 2— 0. 4 mL blood can be collected from the cardiac everytime,and 3—5 times a day. Conclusion Large volume and high quanlity of blood samples can be collected by the three blood sampling methods,which can be used in the rat experiment.

Key words: rat, blood sampling method, many times

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