关键词: 熊果酸, 小鼠肝癌H22 细胞, 抑瘤率, 免疫调节

Abstract: Abstract: Objective The aim of this study was to investigate the antitumor effect and immune regulation activity of Ursolic acid ( UA) on H22 in mice． Method The models of tumor-bearing mice were established by i． p． injection of hepatic cancer H22 and the mice were randomly divided into 5 groups，each groups given by i． p． The tumor inhibitory rate，the thymus index and spleen index were calculated，lymphocyte proliferation of spleenstimulated by ConA and LPS were detected by MTT，T cell subsets CD4 + ，CD8 + were detected by FCM and the levels of The cytokine IL-2，TNF-α， IL-4 in serum were detected by ELISA． Ｒesult In vivo，the inhibitory rate of UA significantly higher than that of the model group． Compared with the model group，spleen index of high dose UA group were significantly lower． The T and B lymphocyte proliferation of spleen were significantly higher． The CD4 + T cell subset and CD4 + /CD8 + T rate were significantly increased，but CD8 + T cells has no significant change． The cytokine IL-2，TNF-α in serum expression were promoted with high dose UA，meanwhile the IL-4 expression was reduced． Conclusion The results show that UA can inhibit tumor growth both in vitro and in vivo． It could play a role of antitumor activity in vivo by regulating the body’s abnormal immune function in tumor-bearing mice．

Key words: ursolic acid, hepatocellular carcinoma cell line of mice ( H22 ), inhibiting tumor rate, immune regulation