人胰岛素基因重组杆状病毒在家蝇中表达的研究

实验动物科学

人胰岛素基因重组杆状病毒在家蝇中表达的研究

李华,董婉维,刘维全,刘海鹏,王本旭,王吉贵,江禹

中国医科大学实验动物部,中国医科大学实验动物部,解放军军需大学军事兽医研究所,解放军军需大学军事兽医研究所,解放军军需大学军事兽医研究所,中国农业大学生物学院,解放军军需大学军事兽医研究所沈阳110001 ,沈阳110001 ,长春130062 ,长春130062 ,长春130062 ,北京100094 ,长春130062

收稿日期:2003-09-20 修回日期:2003-09-20 出版日期:2003-09-20 发布日期:2003-09-20

Expression of Human Insulin Gene with Baculovirus Expression System in Housefly

LI Hua 1, DONG Wan wei,LIU Wei quan 2, LIU Hai peng 2, WANG Ben xu 2, WANG Ji gui 3, JIANG Yu 2

(1. Faculty of Laboratory Animal, Chinese Medical University, Shenyang 110001) (2.The Military Veterinary Institute, Quartermaster University of PLA,Chuangchun\ 130062,China) (3. College of Biology, China Agriculture University,Beijing\ 100094,China

Received:2003-09-20 Revised:2003-09-20 Online:2003-09-20 Published:2003-09-20

摘要/Abstract

摘要： 目的　利用杆状病毒表达系统进行人胰岛素基因在家蝇中表达的研究。方法　用携带有人胰岛素基因的重组杆状病毒感染家蝇幼虫 ,用放射免疫技术、Tricine SDS PAGE和Western blot技术对蝇蛆胰岛素表达水平进行检测。结果　重组的杆状病毒感染家蝇幼虫后 ,蝇蛆中有人胰岛素的表达 ,表达量为 37.4 0 1μIU mL ,占蝇蛆总蛋白的0 .5 83%。结论　利用杆状病毒表达系统可以在家蝇蝇蛆中表达人胰岛素

关键词: 杆状病毒表达系统, 人胰岛素基因, 家蝇

Abstract: Objective\ Study on the expression of human insulin gene by baculovirus expression system in housefly. Method\ The recombinant baculovirous contained human insulin gene weas used to infect housefly larva, the human insulin was detected by Tricine\|SDS\|PAGE and Western\|blot. Result\ Human insulin was expressed by infected housefly larva. And the most expression level was 37.401μIU/ml. Conclusion\ Human insulin could be expressed in housefly larva with baculovirous expression system.

Key words: Baculovirus expression system, Human insulin, Housefly

李华,董婉维,刘维全,刘海鹏,王本旭,王吉贵,江禹. 人胰岛素基因重组杆状病毒在家蝇中表达的研究[J]. 实验动物科学.

LI Hua 1, DONG Wan wei,LIU Wei quan 2, LIU Hai peng 2, WANG Ben xu 2, WANG Ji gui 3, JIANG Yu 2. Expression of Human Insulin Gene with Baculovirus Expression System in Housefly[J]. laboratory animal science.