关键词: 二甲双胍, Ⅰ型干扰素, 糖尿病, 大鼠模型

Abstract: Objective　To investigate the effect of type Ⅰ interferon (IFNα-2b) on the hypoglycemic effect of metformin in diabetic rat models, so as to provide experimental evidence for rational clinical medication.Methods　Sixty SD rats aged 6-8 weeks were used in this study. Among them, fifty-four rats received intraperitoneal injection of streptozotocin (STZ) to establish diabetic models. After successful modeling, 30 rats were randomly divided into 5 groups (n=6) according to the dose of metformin, control group 1, 0.5 g/kg group, 1.0 g/kg group, 1.5 g/kg group and 2.0 g/kg group. Blood glucose changes were observed, and the optimal dose of metformin was determined based on the correlation between blood glucose variation and drug dosage. Subsequently, unmodeled rats were used as the blank group (n=6), and the remaining 24 successfully modeled rats were randomly divided into control group 2, IFNα-2b group, metformin group and IFNα-2b + metformin group (n=6). Rats in the metformin group and IFNα-2b + metformin group were intragastrically administered with 1.0 g/kg metformin. Rats in the IFNα-2b group and IFNα-2b + metformin group were intraperitoneally injected with 4×105 IU/kg IFNα-2b. Blood glucose levels in rats were monitored.Results　The hypoglycemic effect of metformin on diabetic rats showed an obvious dose-dependent manner (R2=0.9109), and 1.0 g/kg was determined as the optimal dose of metformin. After administration, blood glucose levels in the metformin group were significantly lower than those before treatment (P<0.01). However, no significant decrease in blood glucose was observed in the IFNα-2b group and IFNα-2b + metformin group than those before treatment. The differences between these two groups and the metformin group were statistically significant (P<0.01).Conclusion　In the diabetic rat model established in this study, IFNα-2b can significantly inhibit the hypoglycemic effect of metformin.

Key words: metformin, type I interferon, diabetes mellitus, rat model