高凝状态, 小鼠模型, 高脂饮食

Objective To explore the feasibility of high-fat diet feeding KM mice to cause hypercoagulable state of blood in mice, and to provide a suitable and simple animal modeling method for the study of hypercoagulable state. Method KM mice were randomly divided into two groups: control group and experimental group, Control group was fed normal diet ( maintenance diet ) and the experimental group was fed high fat diet ( fat for energy 60. 65%) . On the 30th day, the body weight of the mice was measured and the tail venous blood was collected to determine the activated partial thromboplastin time (APTT ) and prothrombin time ( PT ) of the mice. By comparing the differences in blood coagulation indexes between the control group and the experimental group, it was determined whether high-fat diet feeding could cause the occurrence of hypercoagulable state in mice. Result Under the same feeding conditions and blood collection and hemostasis method, compared with control group, mice in the experimental group were fed a high-fat diet for 30 days, the body weight of mice in high-fat diet group was significantly incread ( P < 0. 001) , and PT and APTT were significantly decreased ( P < 0. 01 ) , with statistical significance, and the blood of mice in high-fat diet group showed hypercoagulability. Conclusion The hypercoagulable state model of mice with shortened PT and APTT can be successfully constructed by high-fat diet.

hypercoagulable state, rat model, hight-fat diets