关键词: C57BL / 6, FVB, 1 型糖尿病, 肠道菌群, 多样性, 16S rRNA 基因测序

Abstract: Objective To compare the composition and diversity of the Gut microbiota of type 1 diabetes mellitus ( T1DM) established by C57BL / 6 ( B6) and FVB mice, and to provide some basic data for further application of the two models in the study of T1DM-related Gut microbiota. Method Forty 8- week old SPF mice were divided into B6 group ( n = 20) and FVB group ( n = 20) , respectively. The two groups of mice were intraperitoneally injected with streptozotocin (40 mg / kg) daily for 5 consecutive days, and fasting blood glucose ≥11. 1 mmol / L for 2 consecutive weeks was considered as T1DM mice. After 6 weeks of modeling, 10 fecal samples were collected from each group, and 16S rRNA gene V3-V4 region was sequenced, the Alpha diversity, Beta diversity, dominant families and the functional pathways related to gut microbiota in fecal were analyzed. Result There was no difference in alpha diversity and richness between B6 and FVB T1DM mice ( all P> 0. 05) , and beta diversity was significantly different in the two groups ( all P<0. 05) . The dominant families of T1DM mice in B6 group were Muribaculaceae, Lactobacillaceae, Lachnospiraceae, Prevotellaceae, Desulfovibrionaceae and Akkermansiaceae. And the dominant families of T1DM mice in FVB group were Lactobacillaceae、Muribaculaceae、Lachnospiraceae、Prevotellaceae、Clostridiales _ unclassified、 Saccharimonadaceae and Marinifilaceae. The proportion of the same dominant bacterial families was a significant difference between the two groups. The ratio of Firmicutes to Bacteroidetes were increased significantly in B6 and FVB groups. The functional pathways of the gut microbiome of T1DM mice in the two groups were concentrated in the metabolic pathways with amino acid, sugar metabolism and nucleotide metabolism. Conclusion There was no difference in alpha diversity of gut microbiome between B6 and FVB groups of T1DM mice, but the composition and proportion of gut microbiome were significantly different. The composition proportion of the dominant gut microbiome was changed in the different models.

Key words: C57BL / 6, FVB, type 1 diabetes mellitus, gut microbiome, diversity, 16S rRNA gene sequencing