关键词: 牛蛙, 蟾蜍, 生理学实验

Abstract: Objective This study explored the feasibility of using bullfrogs to replace toads in physiology teachingexperiments by comparing the experimental effects between bullfrogs and toads in physiology teaching experiments.Method Bullfrogs and toads were separately used to carry out frog heart perfusion experiment, sciatic nervegastrocnemius muscle stimulation and contraction experiment. Result The frog heart perfusion experiment showed that the bullfrogs and toads under the action of Na+ , Ca2+ , K+ , norepinephrine, acetylcholine, lactic acid and sodium bicarbonate had the similiar effect on the activity of the isolated heart. In the sciatic nerve-gastrocnemius stimulating contraction experiment, the stimulation intensity was 0. 1 V caused minimal muscle contraction. Under the stimulation of around 0. 15 V, the muscle contraction amplitude reached maximum and remained urchanged. As the stimulation frequency increased, the skeletal muscles performed single contraction, incomplete tonic contraction and ionic contraction. After stimulated, the action potential conduction velocity of the nerve trunk about bulfrogs and toods is 16. 6 m / s and 13. 16 m / s respectively. There was no essential difference about experimetal results between the bullfrog group and the toad group ( P>0. 05) . Conclusion It is feasible solution that bullfrogs instead of toads to carry out physiology teaching experiments.

Key words: bullfrog;toad, physiological experiment