Abstract: Objective Compared the genetics of the independently established Tg. C57-ras mouse and Japanese Tg. rasH2 mouse model, and the biological characteristics of the first generation of hybrid mice between Tg. C57-ras transgenic mice and BALB / c mice ( CB6F1-NIFDC) and Japanese Tg. rasH2 transgenic mouse model ( CB6F1-CIEA) , which provided theoretical support for applying self-established mouse model in new drug preclinical carcinogenicity evaluation. Method The genetic characteristics, biological characteristics and sensitivity to positive carcinogens of the 2 models and F1 models were analyzed. Result The 2 models was used to construct by the genetically modified method. Analysis of genetics features showed that c-Ha-ras gene fragment has no actual difference, and only 1 copy difference in the copy number. mRNA expression level shows that genes in different organs of the two models are similar. But although the lung tissue protein levels in the Japanese model is slightly higher than the independent model, it reached no statistical difference. According to literature review and analysis, the growth curves of the 2 models were different, but there was little difference in body weight during the carcinogenic experiment cycle. There are some differences in blood physiological and biochemical indexes, and these differences were estimated to be related to genetic background. Conclusion Due to the comparing the characteristic data from 2 aspects, it is suggested that Tg. C57-ras transgenic mice established by ourselves may be a promising candidate animal model for development of a rapid carcinogenicity testing system for preclinical safety evaluation of drugs.