实验动物科学 ›› 2021, Vol. 38 ›› Issue (4): 11-.

• 研究报告 • 上一篇    下一篇

137 Cs-γ 辐照对实验红鲫的组织病理学影响


  

  1. ( 1. 永州职业技术学院,永州 425100) ( 2. 湖南交通工程学院,衡阳 421001) ( 3. 岳阳市妇幼保健院,岳阳 414000)
    ( 4. 南华大学衡阳医学院,衡阳 421001) ( 5. 南华大学实验动物学部,衡阳 421001)
  • 出版日期:2021-08-28 发布日期:2021-10-25

Histopathological Effects of 137Cs-γ External Irradiation on Laboratory Red Crucian Carp

  1. ( 1. Yongzhou Vocational Technical College,Yongzhou 425100,China)
    ( 2. HuNan Institute of Traffic Engineering, Hengyang 421001,China)
    ( 3. Yueyang Maternal and Child Health-care Hospital, Yueyang 414000,China)
    ( 4. Hengyang Medical College, University of South China, Hengyang 421001,China)
    ( 5. Department of Laboratory Animal Science, University of South China, Hengyang 421001,China)
  • Online:2021-08-28 Published:2021-10-25

摘要:

摘要:目的 探 讨137 Cs-γ 辐 照 对 实 验 红 鲫 的 组 织 病 理 学 影 响。 方 法 用 HXFS-IA 生 物 辐 照 仪 对 实 验 红 鲫 进行137 Cs-γ 一次性辐照,剂量分别为 1. 94 Gy、3. 88 Gy、7. 76 Gy 和 15. 53 Gy。 经137 Cs-γ 辐照后的第 14 天,解剖实验红鲫取心脏、肝脏、肾脏、脾脏等组织,按常规方法进行石蜡切片及 HE 染色。 结果 实验红鲫经 1. 94 ~ 15. 53 Gy137 Cs-γ 辐照后,心肌纤维排列紊乱、结构不再完整;部分肝细胞出现水肿,继而出现坏死;脾脏中淋巴细胞逐渐减少,网状细胞和纤维组织增生;肾小管的上皮细胞水肿继而出现细胞坏死,即心脏、肝脏、脾脏与肾脏会随着核剂量的增加,其损伤程度加剧。 结论 在 1. 94 ~ 15. 53 Gy 剂量范围内,实验红鲫经137 Cs-γ 一次性辐照后,心脏、肝脏、肾脏及脾脏均有组织病理损伤。 随着辐照剂量的加大,组织损伤的程度增高。

关键词: "> , 137 Cs-γ, 实验红鲫丨器官组织丨组织病理学

Abstract:

Abstract: Objective To investigate the histopathological effects of 137Cs-γ external irradiation on laboratory red crucian carps. Method The laboratory red crucian carps were subjected to 137 Cs-γ one-time external irradiation in HXFS-IA biological irradiator, with the irradiation doses 1. 94 Gy, 3. 88 Gy, 7. 76 Gy and 15. 53 Gy. On the 14th day after irradiation, these carps were dissected and the tissues of heart, liver, kidney and spleen were taken out. Paraffin sections of the heart, liver, kidney and spleen were stained by HE using routine method. Result The red crucian carps showed some histopathological changes after irradiation from 1. 94 to 15. 53 Gy 137Cs-γ. The arrangement of myocardial fibers is disordered, and the structure of myocardial fibers is uncompleted. Some hepatocytes appeared edema, and then necrosis. In the spleen, lymphocytes gradually decreased and reticular cells and fibrous tissue proliferated. The epithelial cells of the renal tubules were edema, then appared cell necrosis. The heart, liver, spleen and kidneys were damaged with the increase of nuclear dose. Conclusion In the irradiation doses from 1. 94 Gy to 15. 53 Gy, the laboratory red crucian carps were exposed to 137 Cs-γ for one-time external irradiation, and the heart, liver, kidney and spleen got histopathological damaged. The degree of tissue damages with the increase of radiation dose was exacerbation.

Key words: ">137Cs-γ丨laboratory red crucian carps丨organ tissue丨histopathology