Abstract:

Abstract:In this study, a relative quantitative analysis of the sphingolipids in liver homogenate from hyperlipidemia hamsters was carried out by the liquid chromatography-tandem mass spectrometry ( LC-MS) . Principal component analysis ( PCA) and Partial least-squares to latent structure-discriminant analysis ( PLS-DA) were used for data analysis and obvious difference was observed from normal and hyperlipidemia group. Twenty one sphingolipids including sphingomyelins ( SM) , ceramides ( Cer) , and glucosyl-ceramide ( Glc-cer) showed abnormal change in the liver of hyperlipidemic hamsters. Among them 9 ceramides were up-regulated, 4 Glc-cers were down-regulated, while three of eight SMs were up regulated, other five of them were down-regulated. The result indicate the compounds of the SMs-Cers-glycosphingolipids ( GLP ) pathway were disrupted in the model group and it may trigger insulin resistance and inflammation which cause damage on liver in hyperlipidemia. The targeted metabonomic study of sphingolipid pathway based on LC-MS provided more useful information to understand the pathology of hyperlipidemia disease.