Abstract: Abstract: Objective To detect changes in intracellular calcium ion using a detection system of spinning disk confocal microscopy combined with electrical stimulation. Method Calcium ion change is one of the key factors for neuron function. In this experiment, the fluorescent probe fluo-4AM was used to label calcium ions, neurons were excited by electrode stimuli and the spinning disk confocal was used to detect the rapid changes of intracellular fluorescence intensity. Result The electrode was successfully prepared. It was detected that about 10% of the primary neurons had spontaneous calcium ion rise. After electrical stimulation, the intracellular calcium ion rose rapidly. When the stimulation ended, the calcium ion still rise, but the amplitude decreased. Conclusion
Spinning disk confocal microscopy combined with electrical stimulation system detects rapid changes of calcium ions before and after stimulation.