Abstract:

Abstract: Objective To observe the therapeutic effect of hexuemingmu ( HXMM ) tablet on laser-induced choroidal neovascularization ( CNV) in mice. Method C57BL / 6 mice ( aged 8 weeks, male) were selected as experimental animals. CNV mice were induced by double frequence Nd: YAG laser ( wave length: 577 nm; power: 180 mW; duration: 100 ms; spot size: 100 μm; number of light spots: 6. After generation of choroidal neovascularization, CNV mice were randomly   divided into saline group, small dose group, moderate dose group and large dose group. The other operations of the control group were the same as those of the experimental groups except laser exposure. The control group and saline group were gavaged with 10 mL / kg per day of saline. The small dose group, moderate dose group and high dose group were given 0. 34 g / kg, 0. 68 g / kg, 1. 36 g / kg HXMM ( the drug was dissolved in saline) per day respectively. Electroretinogram, fundus fluorescein angiography and choroidal neovascularization staining were performed 1 week after intervention. Result Electroretinogram showed that the amplitude of scotopic 3. 0 response b wave of intervention groups with HXMM was higher than that of saline group ( P<0. 05) . The result of fluorescein angiography in vivo showed that the degree of fundus fluorescence leakage in moderate dose group and high dose group was significantly lower than that in saline group ( P<0. 05) . The result of area of neovascularization measurement was consistent with fundus fluorescein angiography. Conclusion This study confirmed that HXMM has a certain therapeutic effect on CNV mice from aspects of function and morphology, which laid a foundation for further research on related mechanisms.