Abstract:

Abstract: Objective To observe the effect of compound traditional Chinese medicine on lymphocyte function and cytokines of mice infected with influenza virus A / PR / 8 / 34 ( H1N1 ) . Method Ninety BALB / c mice were randomly divided into 6 groups, 15 mice in each group, i. e. normal control group, virus control group, Amantadine hydrochloride group ( 100 mg / kg ) and compound traditional Chinese medicine high dose group (1. 5 g / kg) , medium dose group (0. 75 g / kg) and low dose group (0. 375 g / kg) ,respectively. In the traditional Chinese medicine group, the compound traditional Chinese medicine was administered intragastrically for 2 days, the normal control group was dropped nose with deionized water, the mice infection model was established by dropped nose of influenza-virus in the other 5 groups. Four hours after the model was established, each group was intragastric administrated with different dose of medicine or saline, and continuously given for 5 days. Blood sample was collected aseptically 5 days after gavage. Thymus and spleen were taken after euthanasia, then the thymus and spleen index were calculated. T and B lymphocyte transformation rates of each group were measured by MTT, and the levels of cytokines IL-6, IL-10, TNF-α and IFN-γ in lung tissue were measured by ELISA. Result Compared with normal control group, thymus index and spleen index, T and B lymphocyte A value, the level of cytokines IL-10 and IFN-γ decreased significantly, TNF-α and IL-6 increased significantly (P<0. 01) in virus control group; Compared with virus control group, spleen index and thymus index, T and B lymphocyte A values,cytokines IL-10 in medium dose group and cytokines IFN-γ in high dose groups were significantly increased,cytokines IL-6 was significantly decreased ( P < 0. 05) in medium dose group,cytokines TNF-α level was significantly decreased and IFN-γ was significantly increased (P<0. 01) in medium and low dose groups. Conclusion Compound traditional Chinese medicine can improve lung inflammation by regulating lymphocyte function and cytokine secretion level, which is one of the mechanisms of antiviral activity.